关于赛尔
DESCRIPTION | |
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Catalog No | SRP05449 |
Species Reactivity | Human. Predicted: Rat, Mus. Other species not tested. |
Immunogen | Recombinant fragment corresponding to 57-308aa of human PGK1. |
GeneBank No. | BC113568 |
Alternative Names | PGKA; MIG10; MGC8947; MGC117307; MGC142128; HEL-S-68p |
Purification | Purified by antigen affinity column. |
Isotype | IgG |
Formulation | Lyophilized power with 1% BSA and 0.02% NaN3. |
Concentration | 0.5mg/ml |
Applications | |
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ELISA | 1:20,000-1:80,000 |
Immunohistochemistry | 1:100-1:500 |
Western blot | 1:500-1:3,200 |
For research use only. Optimal dilutions/concentrations should be determined by the final use. |
DATA | |||
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Observed Molecular Weight | 45KD |
PREPARATION AND STORAGE | |
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Reconstitution | If lyophilized, reconstitute in indicated volume of sterile H2O. For extended storage, we suggest the addition of an equal volume of glycerol to make a final glycerol concentration of 50%. |
Storage | Shipped at ambient temperature or with ice bags if in liquid. Store immediately at-20°C upon receipt. Avoid repeating freeze-thaw cycles. |
REFERENCES | |
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Zerrad L, Merli A, Schroder GF. et al. A spring-loaded release mechanism regulates domain movement and catalysis in phosphoglycerate kinase. J Biol Chem. 286(16):14040-14048 (2011) |